Biography of Calamity Jane, Wild West Figure

Biography of Calamity Jane, Wild West Figure Calamity Jane (born Martha Jane Cannary;  1852–August 1, 1903) was a controversial figure in the Wild West whose adventures and exploits are shrouded in mystery, legend, and self-promotion. She is known to have dressed and worked as a man, to have been a hard drinker, and to been skilled with guns and horses. The details of her life are mostly unproven, given the amount of fabrication and hearsay that inform her story. Fast Facts: Calamity Jane Known For:  Hard living and drinking; legendary skill with horses and gunsAlso Known As:  Martha Jane Cannary BurkeBorn:  1852 in Princeton, MissouriParents: Charlotte and Robert Cannary or CanaryDied:  August 1, 1903 in Terry, South DakotaPublished Works:  Life and Adventures of Calamity Jane by HerselfSpouse(s): Undocumented spouses, Clinton Burke, Wild Bill Hickok; documented spouse, William P. SteersChildren: Possibly two daughtersNotable Quote: By the time we reached Virginia City I was considered a remarkable good shot and a fearless rider for a girl of my age. Early Life Calamity Jane was born  Martha Jane Cannary  around 1852 in Princeton, Missouri- although she sometimes claimed Illinois or Wyoming as her birthplace. She was the oldest of five siblings. Her father Robert Cannary (or Canary) was a farmer who took the family to Montana during an 1865 Gold Rush. Jane relayed the story of their journey in her later biography with considerable relish, describing how she hunted with the men and learned to drive the wagons herself. Her mother Charlotte died the year after their move and the family then moved to Salt Lake City. Her father died the following year. Wyoming After the death of her parents, young Jane moved to Wyoming and began her independent adventures, moving around mining towns and railroad camps and the occasional military fort. Far from the ideal of the delicate Victorian woman, Jane often wore mens clothes. She eked out a living doing menial jobs, some of which were jobs usually reserved for men. She is known to have worked on the railroad and as a mule skinner. She worked as a laundress and waitress and may have also worked occasionally as a sex worker. Some legends say that she disguised herself as a man to accompany soldiers as a scout on expeditions, including the 1875 expedition of General George Crook against the Lakota. She developed a reputation for hanging out with the miners, railroad workers, and soldiers- enjoying heavy drinking with them. She was arrested, with some frequency, for  drunkenness and disturbing the peace. Deadwood Dakota Jane spent many years of her life in the boomtown of Deadwood, Dakota, including during the Black Hills gold rush of 1876. She claims to have known James Hickok, known as Wild Bill Hickok, and she is thought to have traveled with him for several years.  After his August  1876 murder, she further claimed that they had been married and that he was the father of her child. (If said child had actually existed, he or she was said to have been born September 25, 1873, and given up for adoption at a South Dakota Catholic school.)  Historians do not accept that either the marriage or the child existed. A diary supposedly by Jane that documented the marriage and child has been demonstrated to be fraudulent. In 1877 and 1878, Edward L. Wheeler featured Calamity Jane in his popular Western dime novels, adding to her reputation. She became something of a local legend at this time because of her many eccentricities. Calamity Jane gained admiration when she nursed victims of a smallpox epidemic in 1878, also dressed as a man. Possible Marriage In her autobiography,  Calamity Jane said that she had married Clinton Burke in 1885 and that  they  lived together for at least six years. Again, the marriage is not documented and historians doubt its existence.  She used the name Burke in later years. A woman later claimed to have been a daughter of that marriage but may have been Janes by some other man or Burkes by another woman. When and why Clinton Burke left  Janes life is not known. Later Years and Fame In her later years, Calamity Jane appeared in Wild West shows, including the Buffalo Bill Wild West Show, around the country, featuring her riding and shooting skills. Some historians dispute whether she was indeed in this show. In 1887, Mrs. William Loring wrote a novel named  Calamity Jane. The stories in this and other fiction about Jane were often conflated with her actual life experiences, magnifying her legend. Jane  published her autobiography in 1896,  Life and Adventures of Calamity Jane by Herself,  to cash in on her own fame, and much of it is quite clearly fictional or exaggerated.  In 1899, she lived in Deadwood again, supposedly raising money for her daughters education.  She appeared at the Buffalo, New York, Pan-American Exposition in 1901, in exhibitions and shows. Death Janes chronic drunkenness and fighting caused her to be fired in 1901 from the Exposition and she retired to Deadwood. She died in a hotel in nearby Terry  in 1903.  Different sources give different causes of death:  pneumonia, inflammation of the bowels, or alcoholism. Calamity Jane was buried next to Wild Bill Hickok in Deadwoods Mount Mariah Cemetery.  Because of her notoriety, her funeral was large. Legacy The legend of Calamity Jane, markswoman, horsewoman, drinker, and performer, continues in movies, books, and television Westerns. How did Jane get the moniker Calamity Jane? Many answers have been offered by historians and storytellers. Calamity, some say, is what Jane would threaten to any man who bothered her. She also claimed the name was given to her because she was good to have around in a calamity, such as the smallpox epidemic of 1878. Maybe the name was a description of a very hard and tough life.  Like much in her life, its simply not certain. Sources Calamity Jane. Life and Adventures of Calamity Jane by Herself. Ye Galleon Press, 1979.â€Å"Calamity Jane: Exposed.†Ã‚  True West Magazine, 21 Aug. 2015.â€Å"Encyclopedia of the Great Plains.†Ã‚  Encyclopedia of the Great Plains | CALAMITY JANE (1856-1903).

Top 3 Ways to Become a Great and Effective Leader

Top 3 Ways to Become a Great and Effective Leader Steps to A Truly Effective Leadership Style Last month I was fortunate to meet a real wonder woman. Not from the DC universe, but the wonder woman from our world. She has three kids, she is married and she runs a bank. Impressive, isnt it? Her name is Gillian and she runs a bank in Toronto. Gillian took the first place in an honorable list of Top 100 Canadas Most Powerful Women, which celebrated spectacular female leaders, who lead by example and inspire thousands of people. From my own experience, I can say that Gillian is really an exceptional and inspiring woman. What I was impressed by the most is her leadership style. Unlike everything you would expect from the bank director, she was authentic, warm and friendly. In our conversation I asked her about her core values and especially her leadership style, which navigated her through the male industry and granted her personal and professional success. We discussed leadership skills and also such important characteristics of leadership as the ability to set right priorities, built relationships based on trust and create a great team. But what was even more important is how a leader can develop his or her own unique leadership style. You may ask, Why is this so important, if the primary ability of the leader is to have vision and lead others towards the higher goal? While this is true, the lack of authenticity usually makes the leader look not trustworthy. So the question is how to be yourself, be a great leader and be a unique leader at the same time? This three-step guide will help you to discover your authenticity and start leading from the heart: Identify Your Strengths. First, you have to understand what your strong points are. Once you know what they are develop them. While working with something that you really like, you will feel engaged and happier. Its also a great way to express your authenticity, because you will project your real desires and motives rather than pretending to be someone else. Alleviate A Key Weakness. Working at what youre good at is not the only thing that leaders do. They also eliminate their weak sides. Unfortunately, this is not something that can happen in a blink of an eye. So, in order to work with your weak sides, you first need to identify them and then work on them for the course of several years. Some common traits that leaders usually need to work on are: being too impatient, too much sarcasm, always being last etc. Admit Your Mistakes. No matter which leadership styles youre utilizing, you need to learn to admit your mistakes in order to become a great leader. If there were no mistakes, there would be no experience. Without experience, we wouldnt know how to move in the right direction. Instead of copying mans leadership style, Gillian developed her own. Why dont you follow her glorious example?

Corporate Social Responsibility (CSR) planning in Toyota Motor in Essay

Corporate Social Responsibility (CSR) planning in Toyota Motor in Europe in 2010 - Essay Example This research will begin with the statement that with the onset of the economic slowdown during the recession period Toyota have gone through several challenges for a sustainable growth in the business environment. In spite of such a tough situation the company has always ensured safety and customer satisfaction to the top priority and implemented several strategies for a better understanding of the market as well as for the environment. After the recession period, the company has gone through a fresh start and steer towards a new growth direction with its new Corporate Social Responsibility (CSR) approach. Always committed to supplying safe and reliable vehicles for the market the company always looked after the dynamic need of the customers and the marketplace together. In the process, the company has great support from the stakeholders and the people related to the company who helped for this sustainable growth of the company in the competitive environment. The company is not only into the development of safer car as its CSR approach but also involved into serving the people associated with the company business who provides the high-quality products from the company for the growing market needs. With the global crisis of energy Toyota also looks after developing eco-friendly cars for the society and grows in hand with the environment with the safe and reliable vehicles for the society. For an efficient handling of the Corporate Social Responsibility program, the corporate team has established the CSR committee who are into the management process of the CSR departments and the promotion of the approach and help the company to implement strategies for its CSR activities. The legal compliance is looked after by the core Corporate Ethics Committee and the Philanthropy Committee manages the social activities for a better reach and promotional activities which facilitates the process of long-term growth for the company. The management of CSR activities for Toyota consistently looks after the improvements of the different processes for the firm. The Corporate Social Responsibility program for the company is primarily focused towards the assessment of the internal as well as the external entities for the firm. The assessment program gives the chance for the company to look after and identify the weak points of the company and evaluate strategies accordingly for the midlevel and long-term growth of Toyota.

Print Ad Case Study Example | Topics and Well Written Essays - 750 words

Print Ad - Case Study Example Being a high-fashion, elite brand, the print ad's target market leans toward relatively young men and women belonging to an upper-class social demographic. Those in its target market have above-average incomes, are brand-conscious, and are generally in touch with the prevailing fashion trends of the day. The brand is all about youth, sexiness, and the brashness that comes with it. Those who wish to portray this sort of image through their fashion style are those targeted by the print ad, and this definitely was definitely illustrated in the edgy, albeit inappropriate theme presented. In developing a new print ad for Dolce and Gabbana, several elements will be modified as the product line will be repositioned completely. From being a high-end, relatively young fashion brand, it is proposed that the new line be packaged as a casual line appealing to an older, more sophisticated demographic comprised of middle-aged, successful professionals . The prevailing theme would be "Sunday Best", and would be presented in such a way that the brand would be their top-of-mind choice for laid- back "country club" weekend wear. The newly repositioned line would be appealing to a target audience that closely mirrors those of Ralph Lauren and Lacoste. The new concept of the reworked print ad will be intrinsically different from the original. The ad is set on what appears to be a country club golf course, and will feature a beautiful, casually dressed woman around her mid-30's as the central character. She appears to be a newcomer to the greens, and is being taught how to swing by a smiling, laid back male, presumably late 30's to early 40's. The man gives off the impression that he is a hard-working professional during the week, say a stockbroker, and he is enjoying the fruits of his labor on the weekend with his beautiful wife. In the background, another affluent-looking couple (presumably friends of the central couple) look on, smiling and apparently amused at the woman's first-time follies at the tee. The tone and mood of the ad is very upbeat and positive, conveying an unspoken vibe of class and sophistication. The Dolce & Gabbana logo will still be superimposed over the print as in the original ad. Consumers will use the product ideally as their primary choice for weekend wear, for light social functions and weekend club meetings. It is a means to identify with the relaxed affluence that is typical of high-income neighborhoods in New York and California. The target market was chosen first and foremost to tone down the controversy generated by the previous print ad. By choosing an older, more affluent target comprised of high net- worth professionals which project much more conservative values, it is hoped that the negative connotation associated with the brand would be eased out of the public's eye. Also, the new target market was also chosen because the company can be sure that there is already an existing market to tap, and that their prices would still seem palatable within the chosen demographic. If the repositioning campaign is rendered properly, Dolce &

Contemporary Architectural Design and Property Development Essay

Contemporary Architectural Design and Property Development - Essay Example In general, a religion means a strict, unwritten code of essential rules (including morals and traditions) established by humans in order to control social life of their society. Vastu and Feng-Shui is a part of old traditions and culture of Chinese and Indian societies. In a time, they became popular in all Asian countries. Both of the concepts refer to the science of planning buildings, travelways, and graves such that they will get maximum benefits and minimum damage from the 'the cosmos'. More specifically, Feng-Shui addresses wind, water, and other natural forces. "Natural forces" in this case include good and bad luck, which are explicitly compared to wind. The idea of both approaches is that inhabitants of a well-sited home, or descendants of someone buried in a good gravesite, can expect wealth, sons, status, and security to flow to them. Rajgopal (2002) explains: The rebirth of Vastu Shastra parallels contemporary spiritual movements arising all over the world that seek to connect with a higher energy, draw closer to the mysteries of the universe, and contribute to a major paradigm shift (p. 33). However, focally, Vastu and Feng-Shui in... s a true folk science, as recognized by the first Western observer to comment extensively on it and by many Chinese and Westerners since Vastu and Feng-Shui also involve an emotional response to landscapes (Freeman, 2005). In India, excellent work is being done which shows not only that modem architecture can be given a worthy landscape setting, but also that it may soon be possible to find landscape architects who can deal imaginatively with the vast new opportunities created for them by modern town and country planning. But no large body of recognizably modern landscape architecture exists, and in only a few countries is there a strong school of designers (Pegrum, 2000). In modern Asian (and Indian) architecture the two great motive forces of the modern movement are on the one hand the new opportunities being created by technical and social progress and on the other the new structural techniques. In landscape architecture new opportunities are certainly being created, but the technique of garden construction is still fundamentally the same as it was in the eighteenth century. "The three main principles of Veda are right orientation, right placement, and right proportion" (Rajgopal, 2002, p. 34). Even the invention of modern earth-moving machinery, which may seem revolutionary, has in fact merely accelerated and cheapened processes which were used by old builders. Rajgopal (2002) explains that: The reason for da Gama's consternation was that all buildings constructed in Kerala, regardless of the faith of their inhabitants, were built according to the principles of Vastu Shastra by takshagans-- skilled craftsmen-carpenters well versed in the ancient science (p. 34). Today, in Vastu and Feng-Shui, in spite of the advance of science and the discovery of new plants,

Cationic Antimicrobial Peptides in Humans

Cationic Antimicrobial Peptides in Humans Antimicrobial peptides Introduction Cationic antimicrobial peptides (AMPs) are gene-encoded peptides of the host defence system made up of 12-50 amino acids, with at least 2 positive charges conferred by lysine and arginine residues and about 50% hydrophobic amino acids (Hancock and Scott 2000). They are produced from gene transcription and ribosomal translation and often, further proteolytically processed (Zhoa 2003). The peptides are folded so that non-polar amino acid side-chains form a hydrophobic face and polar, positively charged residues form a hydrophilic face (Robert and Hancock 1997). Expression of antimicrobial peptides can be constitutive or inducible by infectious or inflammatory stimuli like cytokines, bacteria and lipopolysaccharides (LPS) (Cunliffe and Mahida 2004). They have diverse structures to effectively kill a wide range of microbes at prone sites e. g the skin and lungs, and in secretions such as sweat and saliva (Yeaman and Yount 2004; Santamaria 2005). Many mammalian antimicrobial peptides rous e the hosts innate immune system (Jenssen et al 2006) instead of directly killing the host. Peptides which are found in living organisms from bacteria to plants, insects, fish, amphibians to mammals including humans (Kamysz 2005) are recorded in numerous existing databases e. g. AMSDb (Eukaryotic peptides) (Tossi and Sandri 2002), BAPDb (bacterial peptides), ANTIMIC (natural antimicrobial peptides) (Brahmachary et al 2004) and APPDb. Currently, 1831 peptides are hosted by the Antimicrobial peptide database with 99 antiviral, 453 antifungal, 100 anticancer and 1179 antibacterial peptides (The Antimicrobial Peptide database 2010). In humans, antimicrobial peptides are produced by granulocytes, macrophages and most epithelial and endothelial cells. They boost the immune system, have anti-neoplastic properties and help in regulating cell signalling and multiplication. Amphibian AMPs have been discovered from the skin of frogs from families ranging from Iomedusa, Pipidae, Hyperoliidae, Ranidae, Hylidae, Discoglossidae, Agalychnis and Litoria. The structure of these peptides as unravelled by CD spectroscopy, NMR spectroscopy and molecular modeling (Suh et al 1996) have been found to be generally 10-46 amino acid residues long (Rollins-Smith et al 2005), mostly linear and simple-structured, (Conlon et al 2004) the majority being hydrophobic, cationic and possessing an amphipathic a-helix in nature. Following production, they are stored in the granular glands (poison glands) of skin dermal layer to be secreted in response to injury (Bovbjerg 1963), or as defence against pathogenic bacteria, fungi, viruses and parasites. Biologically active molecules including antimicrobial peptides are produced as large proteins harbouring a signal and an acidic propiece which get cut off to give an active peptide prior to or at secretion from the poison glands (Amich e et al 1999). Cationic peptides are also expressed in the gastric mucosa cells and in the intestinal tract (Kamysz 2005). The best-known peptides isolated from frogs are caeruleins, tachykinins, bradykinins, thyrotropin- releasing hormone (Barra and Simmaco 1995), brevinins, esculentins, magainins, ranatuerins and temporins (Conlon et al 2004). In the past, peptides were extracted using solvents like methanol or acid from the skins of amphibians after sun-drying but with concomitant dwindling of many frog species, other alternative techniques have emerged, one of which comprises stimulating the frog using mild electricity and collecting the skin secretion; 2-4 weeks after, the secretion can be re-collected after replenishment of the glands (Barra and Simmaco 1995). Large amounts of small peptides and their analogues which are resistant to protease cleavage and contain D-amino acids can be chemically synthesised while larger peptides can be expressed in a prokaryotic host from cloned cDNAs coding for a fusion protein (Piers et al 1993). An efficient means of producing therapeutic peptides in transgenic mice red blood cells has been explained by Sharma et al (1994) whereby the required peptide is collected from proteolytic cleavage from the fusion protein where the peptide is at the C-terminal end of human a-globin. Structure of antimicrobials In spite of sharing similar features, sequence homology between antimicrobial peptides is low and secondary structure diversity is wide (Jenssen 2006). Secondary structures based on sequence homologies, 3-dimensional structures and functions can be grouped into 5 classes: Linear, mostly a-helical peptides lacking cysteine, with or without a hinge region (cecropins, magainins). Antimicrobial peptides with one disulphide bond that form a loop structure with a tail (bactenecins, esculentins). Antimicrobial peptides with at least two disulfide bonds yielding primarily or solely a Î’-sheet structure (defensins, protegrins). Linear peptides comprising an unusual composition of regular amino acids with the absence of cysteine (histatins, indolicidin, temporins). Antimicrobial peptides derived from larger peptides or proteins with other known functions (lactoferricins, MUC7, Casocidin1). Mode of action of peptides Unlike numerous antibiotics or secondary metabolites that halt microorganisms over a number of days by hindering the action of key enzymes, most of the vertebrate antimicrobial peptides neutralise microbes quickly by disrupting the membrane or permeating it and targeting anabolic reactions (Barra and Simmaco 1995). The antimicrobial properties of most peptides rely on the formation of a-helical (Oren et al 2002) or Î’-sheet-like tubular (Fernandez-Lopez 2001) structures when interacting with negative charges on cell surfaces or when forming a-helical bundles following self-association in solution (Avrahami and Shai 2002). The mechanism of action of antibacterial peptides is thus twofold: membrane acting (membrane permeabilisation) or non-membrane acting (affect vital intracellular processes) as depicted in Figure 3. Different models of membrane permeabilisation exist; in one model, peptides reorient to cross the phospholipid bilayer of the membrane as a cluster without assuming any particular direction, resulting in a micelle-like complex of peptides and lipids. The toroidal pore model suggests that peptides enter the bilayer at 90 degrees to it, the hydrophilic regions interacting with phospholipid head groups and the hydrophobic regions interacting with the lipid core. Additionally, the membrane curves inward so that the bilayer lines the pore too. The barrel-stave model involves insertion of peptides perpendicularly to the bilayer, forming the staves in a barrel-shaped cluster, such that hydrophilic regions of the peptides project into the pore lumen and the hydrophobic regions contact the lipid po rtion of the bilayer membrane. According to the carpet model, peptides group together parallel to the lipid bilayer and local areas are coated like a carpet (Lai and Gallo 2003). Buforin II in frogs crosses the bacterial membrane without causing permeabilisation and binds DNA and RNA in the cytoplasm of E. coli (Park et al 1998). Antifungal peptides have been found to function in different ways one of which is fungal cell lysis e. g. frog Magainin targeting C. albicans (Tytler et al 1995; Zasloff et al 1987) and frog Brevinin-1 acting on Batrachochytrium dendrobatidis as described by Rollins-Smith et al (2002). Defensin from mammals permeabilises the membrane of C. albicans (Lehrer et al 1985; Patterson-Delafield 1980); bovine Indolicidin has been found to act similarly on T. beigelii (Lee et al 2003). Another strategy of antifungal peptides is the disruption of synthesis of fungal cell wall (De Lucca and Walsh 1999). Cecropin from insects as studied by De Lucca et al (1998) binds to membrane cholesterol or ergosterol and affects the fungus Aspergillus fumigates. Bovine lactoferricin and the hybrid peptide of Helicobacter pylori ribosomal protein L1 and magainin-2, HP(2-9)-MA(1-12), have been shown by Bellamy et al (1993) to cause extensive damage to the ultra structure of the cell wall of C. albicans . Yet a nother mode of action has been documented which involves depolymerisation of the actin cytoskeleton illustrated by Pn-AMP 1 from plants which attacks S. Cerevisiae and C. albicans actin. In contrast, as reported by Helmerhorst et al (1999) and Kavanagh and Dowd (2004), Histatin from human primates acts on mitochondria in C. albicans. Rollins-Smith et al (2002b) found that the peptides magainin I and II, PGLa, and X. Laevis CPF, Phyllomedusa sauvagii dermaseptin and R. catesbeiana ranalexin were more potent at the zoospore transmission period of the chytrid fungus life cycle than against mature stages. It has been found that peptides with primarily antifungal activity particularly abound in polar and neutral amino acids, pointing to a structure-activity link (Lustig et al 1996). Numerous studies point to different modes of action of antiviral peptides which interfere with adsorption, entry of the virus into the cell (Belaid et al 2002) or act on the viral envelope (Robinson et al 1998). Targeted viruses are notably enveloped RNA and DNA viruses except non-enveloped adenoviruses (Bastian and Schafer 2001), echovirus 6 (Pietrantoni et al 2006) and feline. Sinha et al (2003) found that rabbit a-defensin NP-1 acts by inhibiting HSV movement from cell to cell. By interacting with the CXCR4 chemokine receptor functional as a coreceptor for HIV-1 entry into T cells (Murakami et al 1991), a polyphemusin analogue, T22 reportedly inhibits the HIV strains using that chemokine receptor (Tamamura et al 1999). Antivirals can also hamper viral entry through interaction with viral glycoproteins as in the case of ?-Defensin (retrocyclin 2) claimed by Yasin et al (2004) to protect cells from HSV-2 infection by acting on HSV-2 glycoprotein B. Viral antimicrobial peptides can a ct in the cell as well, stimulating genes or proteins, thereby affecting host cell antiviral mechanisms (Bowdish et al 2004);viral gene and protein expression (Wachinger et al 1998) can be hampered too. Antimicrobial activity of peptides Features of antimicrobial peptides The emergence of resistant bacterial strains worldwide necessitates (Bonomo 2000) development of new families of antibiotics. Antimicrobial peptides have properties which render them attractive for development of new antimicrobial therapies. Antibiotic-resistant bacteria are often vulnerable to antimicrobial peptides (Jacob and Zasloff 1994; Tencza et al 1997); minimum inhibitory concentrations (MIC) of 1-8 Â µg/mL competitive with the most powerful antibiotics against resistant organisms have been recorded with peptides (Isogai et al 2009). Lui et al (2009) observed strong effectiveness of peptide nanoparticles against bacteria, Streptococcus haemolyticus (gram-positive bacteria), Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis and also against drug-resistant bacteria methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus, yeast tropicalis and fungi, Candida albicans, Cryptococcus neoformans and Stachybotrys chartarum. Antimicrobial pe ptides not only eradicate bacteria rapidly and neutralize endotoxins but are synergistic with antibiotics and active in animal models (Hancock and Scott 2000). Furthermore, they exhibit a broad spectrum of activity by virtue of their relatively non-specific mode of action. In addition, mutations leading to classical antibiotic resistance do not affect them and they do not easily select antibiotic resistant variants (Shai 2002; Huang 2000). However, peptides have drawbacks limiting their use e. g. they are cleaved by proteases notably trypsin-like proteases and researchers still need to probe further into uptake mechanisms into different tissues (Boman 1995). Activity of Frog peptides Numerous studies aimed at understanding further antimicrobial expression of antimicrobial peptides have been conducted. In line with a first line of defence to the frog, release of antimicrobial peptides are stimulated in response to an environment conducive to microbial proliferation; it has been observed that while the freeze-tolerant wood frog, Rana sylvatica, shows no signs of any antimicrobial peptide activity in winter, it produces and secretes an active peptide upon exposure to warmer conditions (Matutte et al 2000). Rollins-Smith et al (2002a) found that effectiveness is enhanced when mixtures of peptides rather than single ones are released; magainin II and PGLa, synergistically hamper growth of B. dendrobatidis and B. ranarum on the skin of X. Laevis. Frog peptides are seen as interesting and potentially useful molecules that could be effective against a range of human pathogens (VanCompernolle et al 2005), viral, bacterial or fungal. In the 1960s, a 24 amino acids long antibiotic peptide bombinin secreted from the skin of the frog Bombina variegata was isolated but discouraging high haemolytic activity restricted applicability (Csordas and Michl 1970). Since then, large numbers of various peptides have been discovered with antimicrobial potential; Gaegurin, for instance, from a Korean frog is described as having a considerable spectrum of activity with mild haemolytic activity, rendering it a potential antibiotic (Suh et al 1996). Recently, high amounts of peptides were discovered in Litoria chloris, the Australian red-eyed tree frog which blocked HIV without harming T cells; the peptides appeared to target the HIV virus probably by insertion into its outer membrane envelope and punching holes in it (VanCompernolle et al 2005). S ome frog peptides have aroused interest as potential agents for treatment of Type 2 diabetes as exemplified by a peptide isolated from the skin secretions from the leaf frog, Hylomantis lemur with the ability to release insulin from the rat BRIN-BD11 clonal B cell line; A synthetic version of the peptide, phylloseptin-L2 was remarkably good at that. Other peptides from phyllomedusid frogs are a 13 amino-acid-residue peptide from Agalychnis calcarifer (Abdel-Wahab et al 2005) and peptides from Agalychnis litodryas (Marenah et al 2004a) and Phyllomedusa trinatis (Marenah et al 2004b) with a structure similar to dermaseptins. Therapeutic applications Antimicrobial peptides are appealing for therapeutics since they are rapidly produced at low metabolic expenses, stored easily in abundance and readily available shortly following an infection, to rapidly counteract a wide range of microbes (Zhoa 2003). The 20 existing amino acids confer tremendous diversity in sequence and structure of peptides, presenting opportunities in creating a whole range of novel drugs (Hancock and Scott 2000) Peptides can be used for battling antibiotic-resistant bacterial infections or septic shock (Finlay and Hancock 2004). Other potential applications include topical applications for preventing sexually transmitted diseases (Rana et al 2006) including HIV/HSV (Reddy et al 2004), Meningococcal meningitis, diabetic wounds e. g. foot ulcers, gastric helicobacter infections, impetigo (Gunaratna et al 2002; Reddy et al 2004), treating eye infections (Migenix 1998). Creams with snails mucin containing antimicrobial peptides are currently marketed for topical applications treating skin infections and acne inflammation (Cottage 2007). Efforts have also been directed at developing magainin analogs into anticancer drugs (Boman 1995). Furthermore, as attempts persevere to alter the immune system of the vectors or their symbionts to confer to the vectors the ability to eradicate the parasites (Ham et al 1994; Gwadz et al 1989), peptide antibiotics are seen as a potential weapon in fighting insect- borne diseases like malaria, trypanosomiasis, and filariasis. Besides using AMP as proteins, genes encoding AMP can be delivered as gene therapy. Genetically altered bacteria making the antimicrobial in situ can be used for targeting pathogens, which is particularly relevant to the treatment of dental caries, Crohn disease, and other disorders in which disturbances in natural microflora play a role and host-microbe balance must be preserved (Palffy 2009). Peptides are also used for food preservation as exemplified by Nisin, produced by certain strains of Lactococcus lactis subsp. Lactis (Joerger 2003). Engineering peptides for enhanced activity Natural peptides do not always possess all the features required to make them suitable therapeutics, validating the need to engineer their primary structure to confer those properties to them (Sarah et al 1999) e. g. stability, reduced toxicity (Won and Ianoul 2009); existing desirable properties e. g. potency, selectivity or specificity of antimicrobial activity can also be strengthened. In cationic AMPs, antimicrobial activity has been ascribed to the net positive charge since the outermost portion of bacterial membranes is negatively charged by virtue of the negatively charged phospholipids (Brogden 2005). Substitution of acidic residues with basic ones, reportedly boosted the potency of LLP1 against S. aureus from the increase in net charge of the peptide (Robert and Hancock 1997). Hydrophobicity (aiding in membrane insertion) and flexibility, driving the peptides change in conformation to allow interaction with the membrane, have also been identified as important (Jenssen 2006). Antibacterial activity of peptides can be enhanced by altering their flexible secondary structures; altering the membrane-associated conformation of indolicidin to bring the N and C termini closer together increased activity against gram-negative bacteria (Ianoul 2010). Oren and Shai (2000) claim that a successful designing strategy in the synthesis of antimicrobial peptides is the incorp oration of non-natural amino acids (such as Ornithine) and the utilization of D-Amino acids with the aim to sustain natural activity and confer resistance against proteases. It has been found that, often, modest alterations to ineffective antimicrobial peptides successfully confer antifungal properties as evidenced by the resulting potent action of magainin analogues coupled with undecanoic acid or palmitic acid in contrast to the native peptide, against yeast and opportunistic fungal infections (Avrahami and Shai 2003) However, often, one roadblock in peptide production is that expression of antibacterial proteins in bacteria may be suicidal to the cells; this could be circumvented by making a fusion protein which is made to accumulate in inclusion bodies at a later stage in growth (Boman 1995). A report of this technique applied on four fusion proteins was recently published (Piers et al 1993). Use of expression vectors in hosts that are insensitive to the peptide antibiotic is also proposed as a solution that has met with success in producing an insect defensin in yeast (Reichchart et al 1992). There are reports of the baculovirus system being used to express a fusion protein cecropin A although with rather low yields, in an insect cell line (Andersons et al 1991). Using the same virus vector in live insects, expression of cecropin about 60 times higher was recorded in the hemolymph. Pseudin antimicrobial peptides Introduction Pseudin-1, Pseudin-2, Pseudin-3 and Pseudin-4 are antimicrobial peptides with structural similarity, which are secreted from the skin of the bright green and pink paradoxical frog from the Pseudidae family, Pseudis paradoxa, inhabiting Trinidad and the Amazon basin (Olson et al 2001). Pseudins, a subfamily of the Frog Secreted Active Peptides (FSAP) are cationic, amphipathic and helical (Olson et al 2001). Pseudin-2, the most abundant and powerful 2685. 4 Da peptide comprises 24 residues (GLNALKKVFQGIHEAIKLINNHVQ). In aqueous solutions pseudin-2 coils randomly while in those emulating the hydrophobicity of the cell membrane e. g. 50% trifluoroethanol/water, it assumes an a-helical conformation (Yasser et al 2008). Antimicrobial activity of Pseudin-2 As testified by Olson et al (2001), Pseudin-2 effective against Escherichia coli (MIC=2. 5 Â µM), Staphylococcus aureus (80 Â µM) and Candida albicans (130 Â µM) and has very weak haemolytic activity against human erythrocytes in contrast to other frog antimicrobial peptides (Colon 2004). Another study by Pal et al (2005) showed that strains of several pathogenic bacteria Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis and Streptococcus were also effectively acted upon by multi-l-lysine-substituted analogues. They also demonstrated that gradually raising the cationicity of pseudin-2 by replacement of several residues with l-lysine in the hydrophilic part of the peptide enhanced the antimicrobial property. An analogue [D-Lys3, D-Lys10, D-Lys14]pseudin-2 in particular, in contrast to others had a significantly increased antimicrobial effect against E. coli and S. aureus and, low haemolytic and cytolytic activity against human erythrocy tes. Replacing Asn with Lys at codon 3 was found to double the antimicrobial activity against E. coli and S. aureus from a reduction in destabilisation of the a-helix besides greater positive charge (Pal et al 2005). Kim et al (2007), in a study attempting to link structure to mechanical action of pseudin-2 in microorganisms and liposomes, suggested that the antimicrobial activity of Pseudin-2 is ascribed to the punching of holes in the target cell membrane through its oligomerisation according to the toroidal pore model in zwitterionic liposomes and the barrel-stave model in anionic liposomes. Pseudin-2 as an anti-diabetic peptide Besides antimicrobial features, Pseudin is seen as promising, prospective insulinotropic agent for treating type 2 diabetes as an incretin mimetic (Abdel-Wahab et al 2008). Yasser et al (2008) demonstrated that Pseudin-2 and derivatives, particularly a [Lys18]-pseudin-2 derivative, enhanced release of insulin from a clonal B-cell line, BRIN-BD11 via Ca2+ independent pathways. The insulin- releasing features of [Lys18]-pseudin-2 are very similar to the gut hormones GLP-1(7-36) amide and GIP (McClenaghan and Flatt 1999) Mutagenesis Site-directed mutagenesis Initially, DNA mutation was based on generation of random mutations in chromosomal DNA using X-rays and chemicals or error-prone PCR which suffered from the major drawback of not targeting the mutation to a specific gene, leading to the emergence of site-directed mutagenesis. This technique has become instrumental for altering DNA sequences in molecular biology and genetic engineering, and for investigating how protein structure relates to function (Zheng et al 2004). In vitro approaches to site-directed mutagenesis can be generally grouped into three categories (Botstein and Shortle 1985):(i) localized random mutagenesis (ii) oligonucleotide-directed mutagenesis (Cosby and Lesley 1997) and (ii) techniques restructuring DNA fragments e. g cassette mutagenesis (Lo et al 1984). Single amino acid substitutions in proteins can be produced by oligonucleotide-directed mutagenesis. One or more amino acids at specific desired locations in the polypeptide chain can be substituted with no length change. Any residue, chemically reactive or inert, buried or exposed can be targeted, and size can be replaced without altering polarity, or polarity without altering size (e. g. aspartate to asparagine). Often, different multiple substitutions are introduced at one specific position or at several locations to study their effect on protein function (Kegler et al 1994). Saturation mutagenesis Using saturation mutagenesis, which often involves oligonucleotide-directed mutagenesis (Zoller and Smith 1982), a library of mutants with all possible mutations at one or more pre-determined key positions in a gene can be created. In conjunction with high-throughput screening, saturation mutagenesis has numerous applications. For example, it has been used in enhancing the stability of the Rhizopus oryzae prolipase enzyme towards lipid oxidation products such as aldehydes (Lorenzo et al 2007) and to increase enantioselectivity of Pseudomonas fluorescens esterase (Park et al 2005). The ligand-binding specificity of the human oestrogen receptor for a resveratrol-like synthetic compound has also been successfully enhanced by saturation mutagenesis (Islam et al 2009). Randomised gene libraries are usually created by replacing part of the parental gene with a synthetic DNA cassette with specific randomised codons (Hine et al 2003). Cassette Mutagenesis is a means of creating a library by targeting a specific site or sites of any length or sequence and replacing them with new sequences; degenerate oligonucleotides are used for inserting the predetermined degeneracy into the peptide. Cassette Mutagenesis involves cutting the original gene contained in a plasmid with two endonucleases at identified, appropriate restriction sites to remove a small section from the plasmid. To make the sequence more amenable to mutations at any region along it, unique restriction sites around 40 nucleotides apart can be inserted into the plasmid (Stryer 2002). The gap is subsequently replaced with a synthetic double-stranded oligonucleotide (the cassette) with sticky ends which is then ligated to the plasmid which now has the required mutation. The new mutated insert allows protein structures or nucleic acid sequences to be studied. Bakir et al (1993) found cassette mutagenesis to be useful for mutating nine single amino acids in the active site of Aspergillus awamori glucoamylase to modify pH dependence of the enzyme and investigate the functions of the mutated residues. Xiang and Sampson (2004) used cassette mutagenesis to screen libraries for establishing specificity of the substrate in a cholesterol oxidase-catalyzed reaction. Oligonucleotide synthesis or screening can be costly for cassette mutagenesis, especially if several parts of a gene are targeted for saturation mutagenesis, since for each region to be mutated, a different batch of oligonucleotide cassettes containing the mutated codon must be produced (Kegler-Ebo et al 1994). Codon randomisation High throughput substitution can be accomplished through randomisation of codons, resulting in a randomised gene library including all possible amino acid substitutions for the target amino acid residue. Conventional codon randomization uses oligonucleotides containing NNN-, NNB-, NNK- or NNS (N: A/C/G/T; B: C/G/T; K: G/T; S: G/C) since each of these combinations code for all 20 amino acids (Patrick and Firth 2003). Creating NNN libraries from oligonucleotides by employing an equimolar mixture of the four nucleotides at each position represents the easiest approach to codon randomisation (Bosley et al 2005) but a sizeable portion of the library contains premature stop codons especially in the event of multiple codon-randomisations. Additionally, the most common protein mutants with Arg, Leu and Ser (with six degenerate codons) are immensely over-represented in contrast to the rarest mutant with Met or Trp at each randomized position (Patrick and Firth 2003). By employing reduced codon sets, the quality and diversity of the protein library can be enhanced. NNB codons have the lowest likelihood of 1 in 48 of coding for stop codon. NNK and NNS codons reduce the over-representation of the commonly-occurring variants (Patrick and Firth 2003). Although NNK and NNS give rise to a similar distribution of amino acids, NNK is preferred for libraries hosted by E. coli and S. cerevisiae by virtue of their favourite codon use but the NNK codon distributes the 20 amino acids unequally and generates 3% termination codons. Neuner et al (1998) mutated codons with dinucleotide phosphoramidite which has the advantage of requiring only seven dinucleotide building blocks to code for all 20 amino acids. Besides being flexible, the technique can be used applied to various hosts with different codon usage patterns. Virnekas et al (1994) have reported use of trinucleotide phosphoramidites as well in random mutagenesis involving chemical DNA synthesis with a yield above 98%. MAX randomisation The MAX codon randomisation method designed and tested on three residues on a synthetic zing finger protein recently by Hine et al (2005) is a superior technique using simple primers efficiently to curtail library size. Using this technique specific synthetic oligonucleotides anneal to a template containing the codons targeted for mutagenesis which are each fully randomised conventionally (NNN)-to select the particular set of desired codons for E. coli. 20 oligonucleotides, each made up of a complementary region and a MAX codon encoding an amino acid, base-pair at each randomised codon. Specific selection primers are designed to cover the 5 region of each target codon and terminate with each specific codon whose presence in the library is necessary; a set of primers is synthesised for each targeted codon. These are hybridised to the template and ligated to give a single strand. 2 extra unique oligonucleotides are needed for serving as primer-binding and restriction sites at the cassette extremities, also ensuring that PCR amplification of only the selection strand occurs. The resulting double-stranded DNA can be subjected to restriction digestion, dephosphorylation followed by ligation into an expression construct or extended to regenerate the complete gene. One advantage of the MAX technique is that, despite the requirement for a large number of primers, their maximum number does not exceed 20 times the randomized codon-number, resulting in a library comprising 8000 mutants with the absence of codon bias and premature termination codons. While randomising NNN and NNG/T codons generates exponentially-rising redundant gene libraries with increasing randomised codons, MAX randomisation generates non-redundant libraries in essence. Amplification bias likely to arise from using PCR to generate the complementary strand can be minimised by synthesising a second strand instead of using PCR. MAX also allows circumvention of the use of a DNA synthesizer and is helpful when medium to large (103-106 variants) libraries have to be screened efficiently (Neylon 2004). Drawbacks MAX is not practical if only one codon needs to be randomized and cannot be used if the number of adjacent codons to be randomized exceeds two. Furthermore, it is a complicated technique (Hine et al 2003). References Abdel-Wahab, L. M. , et al. (2005) Isolation and structural characterisation of a novel 13-amino acid insulin-releasing peptide from the skin secretion of Agalychnis calcarifer. Biol Che. 386: 581-587. Amann and Brosius. (1985) ATG vectors for regulated high-level expression of cloned genes in Escherichia coli. Gene [Online]. 40: 83-190. Available from: http://www. sciencedirect. com/science?_ob=ArticleURL_udi=B6T39-47PH4H8-BJ_user=153063_coverDate=12%2F31%2F1985_rdoc=1_fmt=high_orig=search_sort=d_docanchor=view=c_searchStrId=1259545403_rerunOrigin=google_acct=C000012698_version=1_urlVersion=0_userid=153063md5=af56ff2270eff863a3062bdd46bc9a70 Amiche, M. , et al. (1999) The dermaseptin precursors: A protein family with a common preproregion and a variable C-terminal antimicrobial domain. FEBS Lett [Online]. 456: 352-356. Available from: http://apps. isiknowledge. com/InboundService. do?Func=Frameproduct=WOSaction=retrieveSrcApp=HighwireUT=000082079500002SID=Q2coKoP%40dF7jNOjpLkgInit=YesSrcAuth=Highwiremode=FullRecordcustomersID=HighwireDestFail=http%3A%2F%2Fwww. isiknowledge. com%3FDestApp%3DCEL%26DestParams%3D%253Faction%253Dretrieve%2526mode%253DFullRecord%2526product%253DCEL%2526UT%253D000082079500002%2526customersID%253DHighwire%26e%3DCkCYpwhLs4N6PGgAE_lYJ503Re6_mdcbI8LWGd6UH07UznpX03dChP3eV4e0frXM%26SrcApp%3DHighwire%26SrcAuth%3DHighwiresmartRedirect=yes[Accessed 25th Feb 2010] Andersons et al. (1991) Biologically a

The Persistance of Immigrants in Charles Bowdens Blue :: essays research papers

For many, our goal is to reach the American Dream: a good job, a home we can call our own, and wealth. However, there are others who only dream of it because they are stuck in a hole that restricts them from reaching it. Charles Bowden, author of â€Å"Blue†, takes readers on a journal across the desert to get a better understanding on why people from Mexico risk their lives to cross to the United States. He provides detailed images and descriptions of Mexicans that have lost their lives trying to cross the desert. Along with his friend Bill, they travel across the desert and encounter danger with snakes, the effects of extreme weather conditions, and experiences thirst, hunger, and fatigue. In the beginning, Bowden â€Å"was sitting at [his] desk in September when a news story caught his eye: seven Mexicans had died of thirst east of Yuma and several more had been snake bitten† (Bowden). To his dismay, the incidents were not treated as important matters because they were from Mexico. What captives the author is that Mexicans are willing to risk their own lives to cross over to the United States. In trying to understand this notion, he begins an expedition through the desert and follows the paths Mexicans traveled and the situations they encountered. As their journey begins, they come in contact with a snake but manage to escape death. Soon after, they begin to experience the effects of harsh weather conditions. With heat â€Å"the body temperature soars and the brain seems to cook. The flesh feels electric with pain as each cell screams out its complaint† (Bowden). They finally reach the Republic of Mexico illegally where they meet up with an offic er. Unlike American officers, Mexican officers have a reputation of bending laws and instead of ticketing people, they often ask for money. Ending their journey, they have learned more of what it feels to be a Mexican traveling the desert. Bowden has also decided to write this story about his experiences to give readers an insight on what happens to people who are willing to risk their lives to live the American Dream.

Mean People vs Nice People

Running head: MEAN PEOPLE VS NICE PEOPLE MEAN PEOPLE VS NICE PEOPLE2 In life there are many emotions that one can think of many people often think of the happier emotions before thinking about the darker side of the emotion wheel. Has one ever wanted to run away from a situation not because of their surroundings but possibly because of the mean people standing in front of them? One way to get away from this situation is to nicely thank them and leave or never show up in the first place to the situation as the people may already understanding that the mean people are like that and do not want to put themselves in that surrounding or situation.The situation is clear that in this world there is nice people and mean people and that people may or may not be wired this way and throughout this, we will discover the true understanding of mean people and of nice people and how to better understand people from these points of view. Can you even imagine that you were only judged by nice or mean and nothing else mattered? Not your personality, not who you were, not all you did for the world or your friends, just if you were nice or mean.Some people find it really hard to be nice or to even play nice, they would rather be themselves and be there mean selves then to even crack a smile or show any sort of friendly nature, they just struggle with it. It is like they were born with DNA that have been given mean genes and not friendly or nice genes and want not â€Å"askmen. com†. There has been a new study about mean people and DNA and how it affect people, but it would not be a fair study without all the nice people in the world, the study with over 348 people.This study made a vivid view of what the researchers were studying and if DNA was a bad component and if it really made a true difference in this study and if DNA was at fault and if it was to blame or if there was other possiblities in life to blame. MEAN PEOPLE VS NICE PEOPLE 3 Can mean people fit in any lifesty le, from working professional, to a college professional, to a college student? Many mean people do not even realize they are mean or just brush it off there shoulders and do not onsider themselves mean but others however consider themselves not nice and consider them mean and sometimes cruel. When this survey came along the people who were asked about being mean had realized not only they were mean but were willing to share there experiences with this product to maybe help others. References askmen. com. (n. d. ). Retrieved from http://www. askmen. com/sports/news/mean-people. html (n. d. ). Retrieved from http://fox6now. com/2012/04/18/new-study-indicates-mean-people-are-born-that-way/

The Aeneid essays

The Aeneid essays The Aeneid was a literary epic written by Vergil. The Roman people as their national epic quickly accepted the Aeneid. Vergil's greatest influence was Homer. The Aeneid is about the Trojan hero Aeneas traveling to eventually start the Roman Empire. After the fall of Troy, Aeneas sail for Italy where him and his followers will settle and eventually establish a colony that will one day become Rome. While on route a storm drives his ships to the coast of Africa and Carthage. Dido, the queen of Cartage, welcomes Aeneas and his men. Venus, Aeneas' mother seeks to protect him from Juno, the goddess who is determined to thwart Aeneas's mission to get to Italy. Venus tries to stop Juno from using Dido in any scheme to delay Aeneas's quest. So Venus decides that Dido should fall in love with Aeneas. Venus inflames Dido's heart and her love for Aeneas begins to grow. Dido entertains her new guests at a banquet where with food and wine, she becomes more infatuated with Aeneas. Aenea s in a flashback remembers his adventures from the fall of Troy to arriving in Carthage. Dido kills herself because Aeneas left Carthage and rejected her love and pleadings to remain with him in northern Africa. Aeneas journeys through the underworld with the aid of a Sibyl. Along the way, they encounter many of Aeneas's friends and a few enemies. On many occasions, Aeneas and a friend talk about past adventures. As Aeneas wanders through the Underworld, he notices Dido wandering about. He tries to talk to her, but his words serve no purpose; she flees from him. He then sees the souls of those who died in battle. He sees numerous horrible sights like: Grief, Disease, Old Age, Fear, Hunger, and several others. Sibyl explains why the dead souls behave the way the do. After witnessing all he did in the Underworld, Aeneas finally meets his father Anchises. Anchises tells Aeneas what is to come in the near future. Anchises lists the descendants of A...

pride essays

pride essays This excerpt taken from the play Antigone written by Sophocles has many different implications. If this idea were to be true, this quote suggests that every crime is caused by pride and that if man did not have pride there would be no crimes and all mistakes would be fixed. One is also able to realize that this quote testifies to the idea that all men do make mistakes, but those who fix them are good and those who dont are evil. If this is true than a mans value and character is determined by his actions. All men do make mistakes, but in cases when they are unable to mend them, it does not make them evil. Additionally, what may prevent a man from acknowledging his crime is not only pride, but also the effect it can have on others. In Death of a Salesman, by Arthur Miller, Willy makes a great many mistakes in his life. These mistakes are in all cases repairable but this does not make him an evil man. One of Willys greatest mistakes is that he depends on the company he works for and believes that they will be as loyal to him as he has been to them. It is not just Willys pride that keeps him believing that even if he is not as successful as he used to be that he will get promoted and keep his job. It is also reasonable expectations of the respect that the company should have for him due to the sacrifices he makes for them. By the time Willy realizes this mistake though, it is too late for him to repair it in anyway. He can apologize and regret his mistakes, but he can not change the past. No matter how many apologies Willy may make to his family, and how much he may regret it, it will not change the situation he is in. Willys mistake was having trust and faith and others but this is not something that makes o ne evil. He did try to fix some mistakes in his life, but others he was unable too based upon how it would hurt others. For instance, Willy can apologize to his wife for the unfaithful ...

Read an article in an art magazine then write the summary Essay - 1

Read an article in an art magazine then write the summary - Essay Example However, one of the art directors was not amused with some of the artworks (Akinsha & Alisa 1). The depiction of President Viktor Yanukovych as an avenger and the overall reflection of the vicious peasant uprising of the 18th century were not welcome amongst conservatives in the Ukrainian art world. Another artist corrected foresaw the impending unrest before it happened. Tsagolov’s The Ghost of Revolution explored the struggles between protesters and police while supported by Mykola Ridnyi. Ridnyi displayed police boots roughly sculpted at the PinchkArtCenter and joined other painters in Maidan or Independence Square for street protest. Other anarchist-artists such as Mann and Ivan used rough walls to draw the image of a famous Ukrainian anarchist leader called Nestor Makhno to inspire protests to continue the demonstrations. However, other artists played safe such as Khomenko of the R.E.P group that drew conventional pencil portraits before giving them away. Performances such as the one titled The Kingdom of Darkness is Surrendered that used mirrors compelled police to view at their own reflections (Akinsha & Alisa

Assignment Essay Example | Topics and Well Written Essays - 1500 words - 10

Assignment - Essay Example All this happened as Shiva was at a cave trying to meditate on the loss of his lovely wife. Parvati did most things to win the heart of Lord Shiva, but all went in vain until one day she decided that she would go for a penance into the forest just to win the heart of Lord Shiva. She was devoted and determined to becoming loved by Lord Shiva, who always saw her as a dark skinned lady. Lord Brahma came over to her and answered her prayer. When she went back to Lord Shiva, she was accepted and loved. Later, they conducted a marriage ceremony which was in a ritualistic manner. That was the way goddess Parvati became the consort of Lord Shiva. Shiva’s side to show a symbol of love and affection always depicts Parvati. The patience and calmness in goddess Parvati is said to be the best combination for Lord Shiva’s angry personality. Lord Shiva acts as the lord of the dances among the Hindu. Shiva’s main role is to destroy then later on restore besides saving humanity. Shiva is also depicted as a householder and an ascetic yogi. In addition, Shiva is also Lord of dance (Nataraja). Lord Shiva for a very long time has been known as a destroyer. c) The circular piece of sculpture in Shiva-Lord of the dance refers to the field of dance that he takes over the entire world/ universe (Fisher, 2014). The circular sculpture also helps in identifying the dance in which it dances with the whole world. d) The floor plan of The Chartres Cathedral has a red arrow, which is linear, this shows that the Christians believe in one-way life. That is, human beings only live once. Contrary to the Hindu Culture where they believe there is life after death and life is regarded as continuous or forever. a) Buddhism is not a religion; it practices atheism. It rejects the fact that God exists. To them the person they are worshiping as their god has mysterious stories surrounding his birth. Buddha is portrayed that after his death he came